High Speed 3D In-Vivo Fluorescence Microscopy

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Automated High-speed 3D Multicolor Localization Microscopy

Single-molecule switching nanoscopy (SMSN) provides a powerful tool to investigate structures and dynamics inside the cell at the molecular scale. It is, however, still far from being a routine technology in biology labs. One of the reasons is that 3D multicolor SMSN live-cell imaging still faces significant technical challenges, such as insufficient temporal resolution, high background from ou...

متن کامل

Optimizing 3D multiphoton fluorescence microscopy.

We present a new optimization concept for 3D multiphoton fluorescence microscopy by finding the optimal excitation beam giving rise to the smallest possible light-emitting volume or the highest possible signal to noise ratio (SNR).

متن کامل

Robust signal detection in 3D fluorescence microscopy.

Robust detection and localization of biomolecules inside cells is of great importance to better understand the functions related to them. Fluorescence microscopy and specific staining methods make biomolecules appear as point-like signals on image data, often acquired in 3D. Visual detection of such point-like signals can be time consuming and problematic if the 3D images are large, containing ...

متن کامل

High-Speed, Random-Access Fluorescence Microscopy: 1. High-Resolution Optical Recording with Voltage-Sensitive Dyes

The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal acousto-optic deflectors under computer control. Each scanning point can be r...

متن کامل

High-speed atomic force microscopy.

High-speed atomic force microscopy (HS-AFM) has been developed as a nano-dynamics visualization technique. This microscopy permits direct observation of structure dynamics and dynamic processes of biological molecules in physiological solutions, at a subsecond to sub-100 ms temporal resolution and an ∼2 nm lateral and a 0.1 nm vertical resolution. Importantly, tip-sample interactions do not dis...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2019

ISSN: 0006-3495

DOI: 10.1016/j.bpj.2018.11.067